RNA isolation, cDNA synthesis and RT-qPCR were performed as previously described using published primers5,6,11.
bProteins not previously described in published BALF two-dimensional gel electrophoresis maps.
The pathophysiological course of SIV infection has been previously described in published manuscripts [ 11– 11].
Spinal columns were dissected and decalcified for subsequent cryostat sectioning (10 μm sections) according to previously described and published in-house protocols [ 28].
We also revisited analysis previously described and published by Elango et al. (2009), which demonstrated that high CpG O/ E genes were overrepresented among genes that were differentially expressed between queen and worker castes (Grozinger et al. 2007).
If the authors had chosen an appropriate scale that covered the full range of thickness, they would see the more classic inferior band that has been previously described and published.
The clinical input parameters for the control patient population were obtained by analyzing data collected at the Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China, from January 2008 to December 2011, as part of a clinical economic trial that has been previously described and published.
Custom codes which have not been previously described in the published literature used in this research will be made available by the corresponding author upon reasonable request.
The additive and allele substitution effects for the traits residual feed intake (RFI), percent meat yield (yield), and percent intramuscular fat (IMF) in beef cattle were calculated as previously described and results published elsewhere [ 19, 36, 37].
The right lung was removed and inflated with 4% formaldehyde to a pressure of 20 cm H2O via trachea, and then fixed in 4% formaldehyde for over 24 hours, as previously described in already published articles [ 7, 23- 25].
