Sentence examples for previously described kept from inspiring English sources

Samples for the conventionalized data set were collected from four wild type mice and four conventionalized TM-IEC C1galt1 −/− mice (colonized as previously described) kept in the Gnotobiotic Mice Facility at the University of Nebraska in Lincoln, Nebraska.

The exosome yield was measured with Micro BCA Protein Assay Kit (Pierce) [22] and Vybrand DiI staining (Molecular Probes) as previously described [23] and kept in −80°C until further use.

Wild-type cells and tetO-RSP5/rsp5Δ cells were grown in the absence or presence of 20 μg/ml of doxycycline as previously described and either kept at 30°C or exposed to a 15-min 30°C to 42°C heat shock.

The collagen gels containing the aortic rings were polymerized in cylindrical agarose wells prepared as previously described (4) and kept in triplicate at 37°C in 60 mm diameter Petri dishes (bacteriological polystyrene, Falcon, Becton Dickinson, Lincoln Park, New Jersey).

Samples were frozen in TEST buffer with 6% (w/v) glycerol and 10% (w/v) egg yolk as cryoprotectants, as previously described [54], and were kept in liquid nitrogen until further processing.

DENV-2 Jamaica was expanded in Vero cells [ 47], purified from the culture supernatants as previously described [ 48], and kept frozen at −70°C until use.

Loss of buoyancy due to rearranged gas vesicle gene clusters by IS transpositions was also previously described in Microcystis strains kept in laboratory culture [ 56].

Groups of six fish were put into tanks and kept as previously described [ 61] at nine degree water temperature.

To access the right branch, the transducer was kept in the previously described position to allow the determination of the portal vein diameter [ 6] and was moved approximately one intercostal space cranially and the angle was set to obtain longitudinal image of the portal vein right branch, as suggested in literature [ 7].

The caterpillar S. littoralis (Boisduval) (Lepidoptera: Noctuidae) and the solitary endoparasitoid C. marginiventris (Cresson) (Hymenoptera: Braconidae) were reared as previously described [ 29]. Adult parasitoids were kept in plastic cages at a male/female ratio of approximately 1 2 and were provided with moist cotton wool and honey as food source.

JEKO-1 cells were kept in RPMI, 10% FCS as previously described.