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Osmotic shock has been previously coupled to other disruption techniques for lipid extraction and Ca2+ signaling response studies in Chlamydomonas reinhardtii, but otherwise has limited utility (Yoo et al. 2012; Bickerton et al. 2016).
Katz et al. reported on a biocatalytic fuel cell based on the surface reconstitution of apo-GOx onto FAD, that was previously coupled to a pyrrolo-quinoline quinine (PQQ) relay conjugated to a self-assembled monolayer of cysteamine on gold.
We have previously coupled the immunoblot approach with cell line standards, expressing different levels of SV40 large T antigen (Tag) expressed over a several fold range, to create a method whereby protein expression could be quantified in absolute terms (molecules per cell) and correlated with any other parameter by multi-parametric cytometry of the same cell lines [6].
We have previously coupled Mab A7 with ferromagnetic particles Bio-Mag41000) and reported its usefulness in rectal carcinoma (Kotani, 1995).
This mixing time is 2 orders of magnitude less than in any mixer previously coupled to a mass spectrometer.
The authors hypothesized that if the previously coupled state of sympathetic arousal was inhibited pharmacologically during the process of reconsolidating reactivated memories, the newly re-stored memory would be permanently dissociated from it [ 41].
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This hyperbolic constraint formulation of Maxwell's equations has been proposed previously, coupling the divergence conditions to the hyperbolic evolution equations, which can then be treated with the same numerical method.
Previously, couples could apply for a legal separation in the courts, or seek a church annulment – a complex process that can take up to nine years.
Previously, couples were generally entitled to exclude gains of up to $500,000 when they sold a principal residence in which they had lived two of the previous five years, but for single filers the exclusion was limited to $250,000.
A previously developed coupled oxidation diffusion model was used to study the influence of coating composition, substrate composition and oxidation temperature on the expected life of MCrAlY coatings.
The findings of this work, which show a more complex picture of STP radiochemistry than previously considered, coupled with errors in IARC Monograph 89, may justify re-examination of the radionuclides currently identified on the FDA HPHC list.
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