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Several circumstantial features have previously been used to identify true PPIs from high-throughput experimental data in yeast [17, 23, 24, 25, 26, 27].
The approach is a relatively efficient way to gather the opinions of stakeholders who are spread geographically (Heiervang and Goodman 2011; Evans and Mathur 2005) and has previously been used to identify research priorities in emergencies and unstable contexts (Tol et al. 2011; Evidence Aid Priority Setting Group 2013; Woodward et al. 2016).
Homology-based BLAST methods have previously been used to identify possible microneme proteins in apicomplexan parasites [82].
The use of transcriptional profiles in combination with metabolic models has previously been used to identify signature pathways in yeast [4] and in human tissues [5].
NRLS data have previously been used to identify other high-risk, low-frequency, events.
In-silico approaches have previously been used to identify novel variants of qnr genes.
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The bioinformatics approach established previously was used to identify the complete sets of TFs from the annotated proteomes of jatropha, papaya, cassava, poplar, castor bean and grapevine.
In zebrafish, loss of Nodal signalling results in the loss of endoderm and most mesoderm, but despite both single gene and genome-scale approaches previously being used to identify Nodal target genes in zebrafish [ 17, 34] the full range of Nodal target genes are unlikely to have been identified.
The strategy and bioinformatics pipeline established previously were used to identify the complete sets of TFs from the annotated proteomes of B. distachyon (v1.0), maize (v4a.53), rice (v6.0), and sorghum (vSbi1_4), and the partial TF repertoires from barley and wheat using their FL-cDNA and CDS resources.
In current study, a standard enzyme-linked immunoadsorption assay, as previously described, was used to identify the HPV16 L1 seropositivity [ 34].
Orthologous protein sequences from S. bicolor, M. sacchariflorus and M. sinensis previously defined were used to identify nsSNP.
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