Next, a previously developed strategy [ 15] was used to establish a formula to calculate the risk score for every patient based on the expression level of the three miRNAs, weighted by regression coefficient: Risk Score = (0.93 × expression level of miR-675-5p miR-675-5p miR-675-5pon level of miR-652-3p) + (−0.726 × expression level of miR-652-3p miR-652-3p miR-652-3p
The bioinformatics approach established previously was used to identify the complete sets of TFs from the annotated proteomes of jatropha, papaya, cassava, poplar, castor bean and grapevine.
The strategy and bioinformatics pipeline established previously were used to identify the complete sets of TFs from the annotated proteomes of B. distachyon (v1.0), maize (v4a.53), rice (v6.0), and sorghum (vSbi1_4), and the partial TF repertoires from barley and wheat using their FL-cDNA and CDS resources.
Northern blot analysis was previously used to establish the mRNA expression pattern of NDRG2 in normal tissues and it has been shown to have the highest expression in brain, heart, skeletal muscle, kidney and liver and lowest expression in tissues such as colon, spleen, placenta and lung [ 5, 10, 11].
We adapt an existing model 8 that was previously used to establish population-wide coverage thresholds levels of ITNs at which community-level protection is equivalent to or greater than personal protection.
A conversion formula could potentially be used to translate previously established normal reference ranges from the SonoSite into those more useful for the Vscan.
Previously established guidelines were used to interpret the Kappa statistics.
Raw data were imported into Affymetrix (Santa Clara, CA) GeneChip software for analysis, and previously established criteria were used to identify differentially expressed genes (Cash et al., 2006).
Principal component and internal consistency analyses were used to reconfirm the previously established internal consistency of the measures.
