Sentence examples for previously been used to amplify from inspiring English sources

These primers have previously been used to amplify non-ecotropic MuERV U3 regions [ 50].

It was then found that the entire MAT1-1 and MAT1-2 idiomoregionsiofs of A. lentulus could be amplified using the primers Loc1 and AMF3, which had previously been used to amplify MAT regions from A. fumigatus (35).

A PCR assay previously described was used to amplify PR and RT genes [20], [21].

To construct a HxBru-Vif− provirus, a two-step PCR technique, which was described previously [32], was used to amplify a cDNA encompassing a region between ApaI and SalI sites of provirus, in which amino-acids at positions 21 and 22 of Vif were changed to a stop codon.

GeneRacer 5′ Primer, complimentary to the anchor sequence and Rev-Gsp primer designed from the 435 bp laccase sequence described previously [19] were used to amplify the 5′end.

Previously published primers were used to amplify 203 bp in the vacA s region (Table 1).

Previously described primers were used to amplify the −652 6N del polymorphism [37], and the D302H polymorphism [36].

Previously published primers were used to amplify and sequence the 16S marker [32] and the 18S gene [33].

Previously described primers were used to amplify 129 bp and 150 bp fragments of the IDH1 and IDH2 genes [ 19].

Polymerase chain reaction (PCR) was performed for all E. cloacae for detection of IntI 1. Koeleman's previously described method was used to amplify the target genes [ 14].

Previously described PCRs were used to amplify a 420-bp fragment of the 16S rRNA gene, a 620-bp fragment of the GroEL gene, and a 300-bp fragment of the Ehrlichia spp. sodB gene (2, 10, 11 ).