Sentence examples for previously been maintained from inspiring English sources

Each sample used six to eight day-old yw females that had previously been maintained on a normal or fasting diet for 48 hours.

All fish within each spawning group were from different families, having previously been maintained in separate compartments and were sized matched using wet weights.

If the alleles that fix had previously been maintained by balancing selection, evolutionary responses will be influenced, at least in part, by how standing variation is maintained.

In 6/15 patients it was possible to show autologous recognition and equal recognition of their stored leukaemia cells, even when they had previously been maintained in in vitro proliferative cultures in liquid suspension and undergoing myeloid maturation for one week.

With an increase of the upper potential limit to 1.23 V vs. RHE, the trends observed previously were maintained but 3D Ostwald ripening strongly overlapped with the three other degradation mechanisms, precluding any identification of the dominant mechanism.

In reality, it is usually important that the results be comparable to those of other studies and that an instrument that has been validated previously be maintained; therefore, repeatedly changing tests would be inappropriate.

C57BL/6 mice were from CLEA Japan Inc. Clec7a (Dectin-1) −/−, Irf3 −/−, Irf5 −/−, Irf7 −/−, Rag1 −/−, and Myd88 −/− mice as described previously were maintained on a C57BL/6 genetic background (Sato et al., 2000; Honda et al., 2005; Takaoka et al., 2005; Honda and Taniguchi, 2006; Saijo et al., 2007; Kano et al., 2008).

If poor nutritional habits have been established previously and are maintained during pregnancy, the pregnant adolescent and her fetus are at increased risk.

HSF1 wild-type and knockout mouse embryonic fibroblasts (MEF) were described previously and were maintained in d-MEM with 10% FCS.

U2OS (human osteosarcoma), COS7, and RDG3 cells described previously [18] were maintained in DMEM (Gibco, Grand Island, NY) supplemented with 10% inactivated fetal bovine serum (Sigma), 1% (v/v) penicillin and streptomycin (Sigma) at 37°C in 6% CO2.

The semi-quantitative grades previously assessed were maintained for the statistical analysis.