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GAIA has previously been employed to study the effect of the lower atmosphere on the upper atmosphere (e.g., Jin et al. 2012; Liu et al. 2013; Miyoshi et al. 2017).
This approach was based on a well characterised method which had previously been employed to study the topology of membrane proteins resident on various cellular organelles including the Golgi apparatus [20].
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The σ RNAP has also been employed to study the kinetics of RPo formation using the previously discussed CoSMos study by Friedman and Gelles.
Structure-activity relationship (SAR) studies have previously been employed to delineate the potential targets of VPA (Bialer et al., 2010; Eickholt et al., 2005; Eikel et al., 2006; Eyal et al., 2005; Shimshoni et al., 2007).
Several methodologies have previously been employed to increase sensitivity.
However, RNA-Seq has not previously been employed to investigate N-deficiency-induced genes.
C. muridarum has a shorter development cycle (∼27 h), and has previously been employed in several studies to understand cellular and molecular aspects of chlamydial infection of human cells [21], [22], [23], and pathogenesis and host response to C. pneumoniae infection [24], [25], [26].
Culture of this cell line has previously been employed by several studies to investigate nephrotoxicity of various toxic compounds [ 13– 15].
These definitions have previously been employed for studies of this type [ 18].
Previously, mainly animal models have been employed to investigate mucosal responses to microbes.
MyoD-Cre and Tie2-Cre reporter mice have been previously employed to study the contribution of endogenous myogenic and vascular progenitors in models of heterotopic bone formation [ 10].
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