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We focus on the problem of mean value drifting found in previous published methods and design our new approach to solve it.
Ergosterol measurements were made according to previous published methods [ 52].
This is more stringent than previous published methods.
The phosphorylation assay was mainly performed according to the previous published methods [ 13, 21].
However, the previous published methods to find gene used bio-experiment or the characteristics of protein sequence.
Clinical disease was evaluated using a subjective scoring method similar to previous published methods [ 34, 38] with minor modifications.
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The salt gradient applied in this study was different from our previous published method and was applied in steps of 0, 15, 15, 20, 25, 30, 35, 40, 45, 50, 57, 64, 90, and 700 mM ammonium acetate in 5% acetonitrile (ACN) and 0.1% formic acid.
Basal bodies were purified following previous published method (Snell et al, 1974; Keller et al, 2005).
We used previous published method to identify AS events in a genome-wide way [ 25].
The protein carbonyl contents were measured according to a previous published method with minor modifications [ 18].
A detailed characterization of the MS standard protein was performed using our previous published method [ 9].
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