Chloride-induced vasoconstriction which appeared to be specific for the renal vessels was potentiated by previous salt depletion and related to tubular Cl− reabsorption [30].
In previous experiments, depletion of Anx2 in HIV-1 infected macrophages not only impaired Gag processing but also reduced virus production [9].
Second, previous Rad21 depletion studies had reported that in the cells with separated sister chromatids, a mitotic checkpoint became activated, preventing those cells from exiting mitosis [44].
Previous double depletion of BIG1 and BIG2 resulted in the dispersion of the TGN with no apparent changes in the Golgi morphology [50].
In accordance with previous reports, depletion of Drp1 by siRNA was found to result in mitochondrial elongation.
In our previous studies, depletion of intracellular ATP retarded the repair of the potential DNA lethal damage [ 6].
Consistent with our previous findings, depletion of Ago2, Dicer or Drosha/DGCR8 impaired HR to a similar degree as Rad51 knockdown.
Consistent with our previous results, depletion of wild-type DAPK2 without or with overexpression of kinase-dead DAPK2, using siDAPK2 3′UTR, led in both cases to a significant increase in ROS.
Thus, in accordance with our previous conclusions, depletion of eIF4G1 in cells lacking eIF4G2 leads to a marked reduction in the rate of translation initiation, but one less severe than that provoked by a comparable depletion of eIF3 subunits [ 8]. (Henceforth, rather than referring to depletion of eIF4G1 in the degron mutant lacking eIF4G2, we describe it more simply as depletion of eIF4G).
In a previous study, depletion of TACC3 led to mitotic arrest and cell death, which was accompanied by apoptotic features, such as Annexin V binding and caspase-3 activation, and the Aurora kinase inhibitor VX-680 increased proteolytic cleavage of procaspase-3 and PARP-1.
