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Bad typography prevents reading; and bad transitions from one screen of text to another can be confusing, or can lose a player's place in the story.
There was an error in the initial release of iOS 8 that prevents reading list pages from opening when offline.
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Allowing only a single mismatch during alignment prevents reads from aligning if both alleles are not considered.
According to a study conducted by the Center for Evaluation, American Academy of Arts and Sciences, reading four or five books during the summer can prevent reading achievement losses among elementary school students that might normally occur then.
Poor quality or low-quality tails prevent reads from being properly mapped.
The special character # separates the reference sequence and the bubble branches to prevent reads from being aligned across the sequence and bubble branch boundaries.
To do this, it is best to locally assemble the reads covering these regions, as SNPs or short variants with respect to the reference that would not prevent read mapping might impede primer binding.
A limiting factor in these studies, however, is rapid virus evolution and resulting polymorphisms, which prevent reads from aligning readily to commonly used virus reference genomes, and, accordingly, make virus integration sites difficult to detect.
These settings allow for sequence variability/sequencing error within populations while still preventing reads from aligning to the incorrect clade, and in practice we found these settings were sufficiently permissive to allow all reads from these loci to match (i.e., there was no substantial increase in the number of mapped reads with more permissive settings).
A polyadenylation signal on the EFGP-Luc cDNA prevents read-through transcription of downstream sequences and therefore its insertion inactivates the targeted c-Myc allele.
The pSAMBt mariner transposon that was designed for essential gene identification has 1) Illumina P7 sequencing adapters (P7) near inverted repeats that facilitate sequencing of mutants and 2) a two-hairpin motif downstream of the ermF cassette that prevents read-through of the transposon disrupted gene [ 14].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com