Both vectors integrate into the same locus (AOX1), and therefore by using Δku70, which prevents off-target integration, a higher proportion of clones with the correct integration should be observed (Naatsaari et al. 2014).
DnaK prevents off-pathway reactions or stabilises certain folding intermediates.
However, kinesin-1 dimers only walk along a single protofilament because their short neck-linker prevents off-axis stepping (Brunnbauer et al., 2012).
In addition, we have observed that one sgRNA does not trigger mutations in Cas9 nickase transgenic flies, suggesting that the Cas9 nickase system significantly prevents off-target effects.
They cite his apparent determination to prevent "off message" members of his party running for important posts, such as Mayor of London or chief minister in the new Welsh assembly.
In trying to prevent off-balance-sheet lending, regulators may push credit off-off-balance-sheet.Shadowing China's banks is not the role the country's policymakers envisioned for the trust companies, says Jason Bedford of KPMG, a consultancy.
Hand-operated screw presses were developed for stamping the designs on the blanks; although the blanks originally were centred on the lower die by eye, it soon became clear that a locating collar would prevent off-centre striking.
Why hasn't the FDA's power to prevent off-label prescribing been challenged?
Such a scenario defines pedagogically sound structures that prevent off-task behavior and engage students in more meaningful interactions.
The result suggested that the addition of EDTA + tannic acid or EDTA + tannic acid + lecithin + α-tocopherol could prevent off-odour, mainly rancidity in shrimp oil-in-water emulsion effectively.
It remains controversial whether the chaperonin system functions solely as an infinite dilution chamber, preventing off-pathway aggregation, or actively enhances folding kinetics by modulating the folding energy landscape.
