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Both the rapid evolution of viruses and the necessity of precise gene regulation could have worked as selective pressures towards the modern miRNA pathway since the requirement for a high degree of complementarity between plant miRNAs and their targets can act as a stabilizer, preventing sequence drift even over long periods of evolutionary time [ 43].
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With this modification, 5′ RNA adaptors can only ligate to RNA molecules without a cap structure but with a monophosphate at the 5′-end which are the typical products of small RNA-guided cleavage, thus preventing sequencing of full-length mRNAs with a cap structure.
Prime series producer Kensuke Tanabe asked the staff to resolve most of the glitches for the Trilogy release to prevent sequence breaking.
The European PAL version resolved these glitches and contained altered elements of the gameplay to prevent sequence breaking, a slower loader that prevented the occasional crashes, slightly different story details, and narration in the opening and closing scenes.
To prevent sequence effects, we used a between-observers design.
The exchange of highly similar sequences between strains would prevent sequence analysis revealing the full extent of horizontal exchange.
The short length of the reads that result from high-throughput pyrosequencing coupled with the high degree of nucleotide conservation among the 16S rRNA genes prevents sequence assembly.
The alignment was trimmed to aligned sequence containing no more than 50% gaps and sequences were removed so that no sequence pairs with an identity higher than 95% remained in order to prevent sequence bias.
We found that clusters of differentiating sites prevented sequence reads from an alternate allele from aligning to the reference genome, causing a bias in relative ASE favoring the reference allele.
Gene trees from various species showed that intraspecific duplicates evolve in concert, perhaps through frequent gene conversion, although this does not prevent sequence divergence, especially where structural heterogeneity physically separates a duplicate from its neighbours.
But a number of factors prevent sequencing from reaching its full diagnostic potential.
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