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Furthermore, the location of edelfosine in mitochondria was mediated by lipid rafts, because when HeLa cells were treated with 2.5 mg/ml methyl-β-cyclodextrin (MCD) to deplete cholesterol and disrupt lipid rafts, edelfosine localization in mitochondria was prevented (data not shown).
To investigate the extent of efficacy against a higher dose of soman, the optimal obidoxime treatment (3.1 mg/kg) combined with atropine sulphate was tested against 44 μg/kg of soman s.c. Although this treatment promoted survival, none of the other physiological effects, such as convulsions or seizure development and respiratory effects, were prevented (data not shown).
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Consistent with a previous report [32], our results showed that high numbers of these CD4+CD25+ T cells (6×106) were insufficient to prevent diabetes (data not shown).
Both protocols were efficient in preventing proteinuria (data not shown) and albuminuria (Fig. 5A).
Unlike the catalytic interface, however, the secondary patch is interspersed with residues which, when mutated, had no effect on oligomerisation, and indeed a substantial part of the secondary patch area could be replaced simultaneously without preventing oligomerisation (data not shown).
However, inhibition of caspase-8 did not prevent MIM depolarisation (data not shown) and only partially prevented cytochrome c release.
As positive control we tested H2O2, which significantly increased CM-H2 DCFDA fluorescence and this could be prevented by catalase (data not shown).
A short 19-kDa fragment appeared, resulting from apoptosis, as z-VAD fmk prevented its appearance (data not shown).
IL-6, similar to the neuroblastoma/monocyte CM (Fig. 2), significantly increased NK cell secretion of IL-6, whereas lenalidomide prevented this effect (data not shown).
The modified TE has shown to prevent DNA degradation (data not shown).
Co-implantation with other ectopic tissues did not prevent tumor disappearance (data not shown).
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