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The technology that was developed to prevent variations in urinary catheter use often stripped the users of their tacit knowledge and forced them into decisions based on categories.
TEG® analysis of the individuals was performed on the same day to prevent variations in machine function from day to day.
Images were captured within 20 min of mounting animals on slides to prevent variations in DAF-16A::GFP localization due to prolonged stress induced by mounting.
The measurement was repeated three times, and fluorescence intensities of the cells expressing EN-actin were in each replicate normalized to the controls to prevent variations caused by antibodies dilutions, etc. Results are presented as a mean of three experiments ± standard deviation (SD) and were plotted using Prism GraphPad.
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To prevent variation between conditions, generation of RVF-VLPs, immune precipitation, and immunoblotting were performed for all conditions at the same time.
In order to standardize both procedures and prevent variation in procedures, a protocol has been developed for both surgical procedures.
DNA was extracted using QIAamp DNA blood kit (QIAGEN) under the standardized protocol to prevent variation of DNA quality.
The interview was carried out by the second author of this article only in order to prevent variation in questionnaire administration and ensure standardization.
To prevent variation between different 2DE gels, all volumes of protein spots in the 2DE map were normalized by using the ratio of each spot over the sum.
To prevent variation caused by the on-chip mixing of lysis buffer and sample, mixing of lysis buffer and sample was performed manually before performing chip-based RNA isolation.
We compared the expression levels of the genes to that of the HPRT-1 housekeeping gene for each sample, and all mRNA/HPRT-1 expression levels were approximately one when comparing a pool of cell line cDNA for each experiment to prevent variation between experiments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com