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The culture plates were covered by their lids to prevent medium evaporation.
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The bank said that "some further monetary stimulus" would probably be needed to prevent medium-term inflation from falling below its 2% target.The pound fell to a five-year low against the dollar, after the Bank of England's governor said that Britain was entering a recession.
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In order to counteract the loss of culture volume during the 40-day culture period, the bioreactor was equipped with air humidifier (Drechsel-type washing bottle) and outlet PTFE filter (Fig. 1), which effectively prevented medium evaporation and moisture escape.
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For the laser therapy experiment, we used an IDAS Laser System (WaveLight Co). to provide stable power with a pulse wave to prevent the medium from boiling.
The autoclave tray holding the flask was filled with 1 to 2 in. of water to prevent the medium from boiling out of the Erlenmeyer flask.
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Before performing the cell assay, 5 mL FBS-free DMEM were added to the gel for 1 6 h in order to stabilize the pH, for saturation of the gel and to prevent culture medium from diffusing in the gel during the experiment.
It also prevents the medium from growing in some ways.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com