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The upstream retained region was extended from 6 bp to 100 bp when two neighboring genes were located head-to-head in opposite orientation to prevent deletion of potential promoters for flanking genes.
"We've had robust litigation hold procedures in place from our very first lawsuit to prevent deletion of emails relevant to ongoing litigation," an Uber spokesperson told TechCrunch.
Different natural strains of C. briggsae varied in their susceptibilities to ΔmtDNA accumulation, owing in part to preexisting compensatory mtDNA alleles in some strains that prevent deletion formation.
RelEB was shown, when introduced into the E. coli chromosome to prevent deletion of flanking DNA and thus to diminish large scale genome reduction [ 59].
However, they left unanswered the question of whether T cell help could prevent deletion of B cells activated by previous exposure to self antigen, as may occur during normal B cell development in vivo.
These results are consistent with previous observations of low frequency stable integration by non-homologous recombination and deletion on the ends of the inserted DNA [ 20, 21, 25], underscoring the need to increase the proportion of stable transformants and to prevent deletion of barcodes.
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Cheung et al. proposed that DOG-1 may prevent deletions of G/C tracts by unwinding G-rich secondary structures arising during lagging strand DNA synthesis [ 18].
These results indicate that the iREX can stably maintain the cloned DNA insert by preventing deletion at two homologous sequences.
Proteins of the Bcl-2 family are crucial for the induction of cross-presentation initiated cell death because in a model of self-tolerance in which OVA was expressed selectively in pancreatic -cells, overexpression of Bcl-2 or loss of Bim prevented deletion of CD8+ OVA-reactive T cells, implicating a role of both in mediating cross-tolerance.
Pol γ proofreading 3′→5′ exonuclease activity minimizes the frequency of point mutations and prevents deletions, thereby contributing to the stabilization of mtDNA in yeast cells (Vanderstraeten et al. 1998).
These sequences, present at the ΨND5-2 direpeatepeassociatedted with the deletions, appear to prevent the deletion by reducing sequence homology between ΨND5-2 aND5ND5 and to thereby place an upper limit on the proportion of ND5-deletion bearing genomes able to accumulate within individuals [ 10].
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