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Similarly to our previous studies [7, 11], in order to prevent any effect of the fluorophore conjugation on the binding affinity, both VHHs were site-specifically conjugated to their respective fluorophores using a cysteine that was introduced in the C-terminal region.
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Coding regions were used to prevent any effects from neighboring genes.
To prevent any effects of heteroscedasticity, Greenhouse Geisser corrections were applied to ANOVAs when sphericity could not be assumed (Mauchly's sphericity test <.05).
In further support of the protective role of BH4 against MTX-induced oxidative stress, GTPCH overexpression prevented any effect of MTX on either eNOS activity or superoxide production.
Specifically we compare two scenarios: (1) a "no selection" scenario in which cooperation does not affect individual fitness, which prevents any effect of evolutionary dynamics on demographic dynamics and (2) a "selection" scenario in which cooperation affects individual fitness, which potentially allows evolutionary dynamics to affect demographic dynamics.
Although CNet, the Internet media company, declined to comment yesterday, its network service provider reported detecting an attack on the company's sites Tuesday afternoon but said it had managed to prevent any crippling effect on users.
A source of linearly polarized light of high wavelength (λ = 633 nm) and low power (12 mW) was used in order to prevent any photomagnetic effect.
To prevent any charging effect and obtain images with the highest resolution, each sample was covered with a fine layer of carbon.
Nowadays subsidy payments on electrical energy have to be managed and controlled to prevent any side effect particularly in developing countries.
On the other hand, if the aim is to obtain the intrinsic mechanical properties of this particular sample, mechanical loads ought to be small enough to prevent any substrate effect.
Developing the optimal mix of these tools, including research, public education, media coverage, integrated legislation, will be significant to proactively manage the complexity of nanotechnology and prevent any undesirable effect due to the NMs exposure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com