Loblolly pine chips were pretreated using WEx pretreatment in a 10-l reactor.
For immunohistochemical analysis, 4 μm TMA sections were automatically pretreated using a pretreatment module (PT-Link; Dako, Glostrup, Denmark) and then stained (Autostainer Plus; Dako) with the monoclonal anti-cyclin D1 antibody DSC-6 (Dako), diluted 1 50.
To permit a comparison of IL pretreatment with a conventional state-of-the-art pretreatment method, one of the lignocellulosic samples was also pretreated using classical sulfuric acid pretreatment.
Sections were pretreated using the Paraffin Pretreatment Reagent Kit II (Vysis): the slides were placed in the pretreatment solution at 80°C for 50 70 min and in pepsin solution 0.05% at 37°C for 20 25 min.
Herein, we have assessed plant-expressed CBH as an additive to a commercial cellulase for saccharification of these pretreated bagasse substrates and bagasse pretreated using a novel, acid-catalysed ethylene carbonate (EC)/ethylene glycol (EG) mixture, 1-butyl-3-methylimidazolium chloride (BMIMCl), and ball-milling.
The corn stover was pretreated using a dilute sulfuric acid pretreatment method in a continuous pilot scale pretreatment reactor [ 26].
Corn stover was pretreated using the dry dilute acid pretreatment (DDAP) method (Zhang et al. 2011; He et al. 2014).
Absorbance at 1132 cm-1, which arises from crystalline cellulose [ 41], decreased after pretreatment with acidified EC/EG, while bagasse pretreated using BMIMCL and ball-milling had reduced absorbance at 1107 cm-1.
The pretreated material was assumed to be corn stover pretreated using dilute acid hydrolysis.
The sugarcane bagasse was pretreated using the steam explosion (SE), acid and alkali pretreatment methods.
Three experimental groups were used (Table 1) with n = 5 each as follows: 1) normal control mice under SB-stress and NE-stress ± pretreatment with phentolamine (PHE), an α-adrenergic receptor antagonist; 2) NOS-inhibition mice pretreated using Ng-nitro-L-arginine methyl ester (L-NAME) under SB-stress and NE-stress; and 3) transgenic knockout eNOS−/− mice under NE-stress.
