Sentence examples for pretreated them from inspiring English sources

In order to address these potential confounds, we allowed VTA-PFC-HIPP cultures to develop as usual for 16 25 days, but prior to the electrophysiological experiments pretreated them with a cocktail of reserpine and alpha-methyl-p-tyrosine (AMPT).

Furthermore, when we established HCT116 xenografts in mice, pretreated them with 5-FU followed by systemic administration of MSC.sTRAIL, we saw complete remission of the tumors as compared with partial responses in the cohort treated with MSC.sTRAIL alone.

To further validate PTEN expression mediated bosutinib-rescued tumorigenic phenotype in thyroid cancer cells, we transfected wild-type PTEN in FTC236-PTEN null cells and parallelly knockdown PTEN in FTC133-PTEN wild-type cells, then pretreated them with or without bosutinib before assaying their migration rate.

Thus changes in microstructure can be avoided and makes it also possible to process very thin magnesium-substrates and pretreat them for adhesive bonding.

If your shoes have deep grass, food or oil stains, they'll come out cleaner if you pretreat them with stain remover.

To analyze the binding affinities between the aptamers and the E1E2 glycoproteins of different genotypes (genotypes 1 through 6), 96-well ELISA plates were pretreated by coating them with 100 µg/mL poly-L-lysine.

Sections destined for IL-1α, PNM, Stress Marq, and Aβ immunoreaction were pretreated by placing them in boiling sodium citrate buffer (0.01 M, pH 6.0) for 20 minutes; sections for ApoE immunoreaction were placed in trypsin solution for 10 min at 37°C, and all were blocked using protein block (DAKO), and immunoreacted by overnight incubation at room temperature.

As a next step, we pretreated cells with these constructs, incubated them for 5 days to establish RNAi, seeded them again and transfected them with viral RNAs.

However, it is necessary to emphasise that 54 (25%) of these patients are affected by prostate cancer and 37 (68%) of them were not pretreated with chemotherapy.

To check how ingested adipocyte material influences macrophages in an inflammatory environment, we pretreated macrophages with LPS before adding them to adipocytes.

We further compared the difference in protein levels of IL-6 and IL-8 in MH7A cells pretreated with IL-29, then stimulated them with TLR ligands or cells preincubated with TLR ligands and subsequently stimulated them with IL-29.