To screen for IBV inhibition, cells and virus were pretreated with extracts, followed by infection in the presence of extract.
Four groups of five mice each were pretreated with extracts (250 and 500 mg/kg), diclofenac (1 mg/kg) and distilled water (3 mL/kg).
To test the possible participation of Sia in DENV-mosquito protein interactions, we performed a parallel VOBPA assay, where we pretreated protein extracts from the SGs or saliva with sialidase.
The serum samples were pretreated and extracted by solid phase extraction.
Therefore, coal samples must be pretreated to extract vitrinite components and remove inorganic (mineral) components before testing and analysis.
After incubation, the samples were pretreated and extracted with SPE and LLE and derivatized as described above.
To confirm the specificity of the TRAP assay in zebrafish samples, we pretreated the protein extracts with RNase.
Furthermore, while UVA exposure led to reduction of mRNA levels of γ-GCLC (0.51 ± 0.01-fold decrease; p < 0.001), γ-GCLM (0.59 ± 0.04-fold decrease; p < 0.001) and GST (0.74 ± 0.04-fold decrease; p < 0.001), upregulation of their mRNA was detected in the cells pretreated with AVS073 extracts and GA.
The cells were pretreated by PFSP extracts (0.1, 0.5, 1, 2, and 3 mg/mL for RAW 264.7 and 1, 2, 3, 4, 5, and 6 mg/mL for cancer cells) for 24 h, 48 h, and 72 h subsequently after the cells were washed twice with phosphate buffer saline (PBS).
The kit provides an immortalized human 293 kidney cell extract as a positive control and 293 cell extract pretreated with RNase as a negative control.
