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Mr. Esposito, the presumptive target of the investigation, did a turn instead as a star witness for the prosecution.
We note that E74 was identified in only one of the two immunoprecipitation experiments, justifying the behavioral screen of mutants representing all presumptive target genes.
We investigated the neurite outgrowth functionality encoded by presumptive target transcripts of 63-set miRNAs and were able to support some of our predictions using data from an unrelated siRNA screening project that analyzed genes by high content screening (HCS) for their ability to regulate neurite growth in differentiated Neuro2A cells (Figure S2).
The clustered distribution of these regions along chromosomes and, importantly, around their presumptive target genes suggests that gene regulation involves the coordinated action of numerous, widely dispersed elements.
Also, the involvement of presumptive target genes can only be inferred from the positional comparison of the genomic ratio profiles to the underlying chromosomal matrix.
Of particular importance is the observation that most UCRs are clustered into arrays that span hundreds of kilobases around their presumptive target genes.
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The presumptive targets of their argument are at least some of the modeling projects undertaken in contemporary climate science.
Intriguingly immunoreactive STC did not entirely co-localize with areas of high STC gene activity and instead appeared to accumulate in presumptive targets of the hormone (uterine epithelium, stromal and decidual cells, trophoblastic giant cells).
In fact, presumptive targets of singletons show a similar pattern to the presumptive targets of CRMs, but the levels of expression are lower.
Ultimately, the speculation regarding the roles of all these presumptive targets will be justified only if they are experimentally validated.
There are many possible explanations for the absence of perfect negative correlation between miRNA expression and expression of presumptive targets.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com