To test this hypothesis, presumptive neuroectoderm cells were targeted with DN-Wnt5a, a truncated Wnt5a construct previously shown to inhibit Wnt5a activity (Choi and Sokol, 2009).
The identification of gelsolin actin complexes inside the joint space is strong presumptive evidence of the sequestration hypothesis, as long as the isoform associated with the complexes is plasma gelsolin.
By using gain- and loss-of-function approaches, we tested this hypothesis and found that the presumptive telencephalic cells induce the expression of Ras-dva1 through secreted Fgf8 in cells of the adjacent non-neural ectoderm.
These results lead us to reject the hypothesis that the metathoracic tarsus develops by suppression of a presumptive distal joint.
Although the effects of forskolin on Stage 1 waves are unknown, its presumptive increase of firing pattern in the developing retina, may complement the epibatidine experiment, further supporting the hypothesis that spontaneous activity drives the V1 effect observed.
Saliva samples are being collected for non-presumptive proteomic analysis and plaque samples are being collected for molecular microbiome analysis to address the hypothesis that the nature of the subgingival biofilm may correlate with renal status [ 121, 122].
Early studies using electron microscopy and Golgi staining (Gallego, 1986; Génis Galvez et al., 1977; Hinds and Hinds, 1979, 1983; Prada et al., 1987; Tarrés, 1984) demonstrated that presumptive migrating RINs can have both bipolar or multipolar morphologies, and suggested a number of distinct hypotheses relating these morphologies to RIN type and migratory phase.
Third, a presumptive right primer was designed for the 15 FSTs that did not have MuTIR sequences based on the hypothesis that these MuTAIL products were not completely sequenced.
Chronology overthrows the hypothesis.
