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For representing the selection and mutation pressures, we used a universal approach based on calculating the shifts in the genetic ratios of competing bacterial genotypes within the particular habitats and across several habitats.
To evaluate the structure and stability of G-, F-, and B-actin over a wide range of temperatures and pressures, we used Fourier transform infrared spectroscopy in combination with differential scanning and pressure perturbation calorimetry, small-angle x-ray scattering, laser confocal scanning microscopy, and transmission electron microscopy.
In order to build reliable homology models and analyze the structural context of residues under selective pressures, we used the HHpred web-server (http://toolkit.tuebingen.mpg.de/hhpred) [ 76] to identify suitable templates and obtain their sequence alignment with the target protease protein sequences.
To test whether the III M chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine whether isogenic males that differ only in the presence of the Y M or III M chromosome have different gene expression profiles.
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"The only pressure we used," he said, "was, 'Well, look, if you don't want to co-operate for your own good, we'll just send you over to the Soviet Union.
To maintain the patient's blood pressure, we used a high dose of inotropic agents.
Gentle, gradual pressure we used to compress the anterior abdominal wall, resulting in displacement and compression of normal bowel loops.
We calculated that 53 mmHg was the estimated pressure we used to perfuse each mouse.
To identify functions potentially under selective pressure, we used an innovative approach, focusing on gene pathways instead of outliers.
To further investigate the participation of the renin-angiotensin system in the effect of lead on arterial pressure, we used losartan, an AT1 receptor blocker, and enalapril, an ACE activity blocker.
For blood pressure, we used a recent meta-analysis of the Blood Pressure Lowering Treatment Trialists Collaborationn.
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