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Analgesic activities of the isolated compounds were assessed in rats with hot plate test and paw pressure assay.
The surface pressure assay involved injecting FAPP2 into monolayers composed of POPC and 2% PtdIns(4 P (Fig 4B).
This approach has been successfully adopted in our laboratory to improve antagonistic potency of bradykinin B2 antagonists in the rat blood pressure assay (Lammek et al. 1990 , 1991.
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Blood pressure assays were conducted on female rats (250 300 g) anesthetized with a ketamine-xylazine (Ketamine 80 mg/kg and Xylazine 8 mg/kg) solution and maintained by artificial breathing.
Membrane sheet tubulation and lipid monolayer surface pressure assays were performed as described previously (Cao et al, 2009).
Here we present a pressure shift assay (PressureFluor, analogous to the ThermoFluor thermal shift assay) that uses high pressure to denature proteins.
The pressure shift assay (PSA, also termed either PressureFluor or differential pressure fluorimetry) was used to study the thermodynamics of decanoate and dodecanoate lipid binding to human serum albumin (HSA) in the temperature range from 25 °C to 80 °C and the pressure range from 0.1 MPa to 400 MPa.
In cell pressure probing assays, atpip1 4-3 and WT plants displayed significant differences (P < 0.05) between each other in parameters of water relations except for cell volume and cell surface area (Supplementary Table 2).
In this work, a fraction of the resulting hydrodynamic flow was successfully guided to an electric field-free analysis channel to implement a pressure-driven assay.
All A1C tests at HPMG were done at one accredited clinical chemistry laboratory using a standard high-pressure chromatography assay method (18) with a coefficient of variation of 0.058% at A1C of 8.8%.
All A1c assays were performed at the same centralized, accredited clinical chemistry laboratory using a high pressure liquid chromatographic assay with a normal range of 4.5%to6.1%1% and a coefficient of variation of 0.58% at a A1c level of 8.8% [ 23] Of 610 study subjects enrolled in owned clinics, 517 (84.8%) had at least one A1c test done in the 12-month period prior to the survey.
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