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A comparative study of PRESS sequences at short- (35 ms) and long- TE (97 ms) found long- TE to be superior for the following reasons: 1) it permits a more favorable voxel localization, and 2) it produces a well-defined narrow 2HG signal at 2.25 ppm, thereby leading to improved differentiation between 2HG and Glu, Gln, and GABA signals.
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The PRESS sequence was simulated with NMR-SCOPE.
Five exemplary pulses were implemented into a PRESS sequence and validated by acquiring images of a water oil phantom and lactate spectra at TE = 144 ms.
MRI (magnetic resonance imaging) was performed with a 1.5 T system; spectral acquisition was performed with a single-voxel technique and a PRESS sequence.
The MEGA-PRESS sequence is able to highlight the GSH signal by adding two editing pulses with a normal PRESS sequence.
Full credit (a score of 1) was given for each correct, 5-key press sequence; partial credit was given for sequences with four consecutive key presses (score of 0.8) and three consecutive key presses (score of 0.6).
A PRESS sequence with a TR/TE 2500/22 ms was used and the LCModel was used for analysis.
Data were acquired using a standard PRESS sequence with an echo time of 30 ms, a relaxation delay of 2,000 ms and 176 transients.
First, a standard PRESS sequence (TR = 3 s, TE = 68 ms) was used to acquire an unedited spectrum with 32 averages.
In brief, a voxel of interest was placed over the hand motor cortex in session 1 and the visual cortex in session 2. A standard PRESS sequence was acquired to assess the creatine and NAA line widths, and a MEGA-PRESS sequence was then acquired to allow simultaneous spectral GABA editing, three-dimensional voxel localization, and water suppression [ 23].
For H-MRS measurement of intramyocellular lipid (IMCL), single voxel (2 cm) spectra localised to the right quadriceps muscle were acquired using a PRESS sequence (TR 5,000 ms/TE 30 ms) [ 32].
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