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This peptide inhibited TNFα-induced priming of ROS production while preserving the physiological response to bacterial derived N-formyl peptides.
A 6xHis-tag was added C-terminally to recruit the protein to membranes preserving the physiological orientation.
Thus, leaves play an important role in preserving the physiological normal state of plants during drought stress.
Employing viruses as nanoscopic lipid-enveloped test tubes allows the miniaturization of protein protein interaction (PPI) assays while preserving the physiological environment necessary for particular biological processes.
In intubated COPD patients, PAV improved minute ventilation, decreased dyspnea, and reduced WOB [ 42], while preserving the physiological breath-by-breath VT variability better than PS [ 43].
If this hypothesis is correct, targeting RBPjκ-independent Notch signaling might be a safe but effective approach to block oncogenic Notch activity while preserving the physiological activities of Notch which may be important for normal tissue functioning.
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Nevertheless, to be effective, such cancer surrogates must preserve the physiological relevance of the in vivo models in order to provide realistic information on drugs' efficacy.
Notably, Laverty et al. demonstrated that their method of receptor reconstitution preserved the physiological long-distance crosstalk between different ligand-binding sites: varying the amount of a given ligand bound to the receptor modulated the binding of a different, radioactively marked ligand to a distant site (Fig. 1f and Extended Data Fig. 2d of ref. 1).
In a first step, we approached immunohistochemistry on cryosections that are not only expeditious but also preserve the physiological epitope better than plastic sections [3], [6].
Thus to explore the role of the macrophages in metastatic seeding and extravasation mechanistically, we used an intact ex-vivo lung imaging system that preserves the physiological lung structure [42].
This is in contrast to methods whereby an excess of the PKA catalytic subunit is added to fibers [59], a strategy that is unlikely to preserve the physiological framework wherein changes in PKA subunit expression or phosphorylation state may dictate altered substrate selectivity [50], [60].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com