Following 33-cycle PCR optimised to preserve ratio of amplification of test and reference, the ratio of test to reference was determined by fluorescence quantification of Eva Green binding during real time melting of the amplicons in a 384-well LightTyper (Roche Diagnostics); test and reference amplicons were designed to melt at different temperatures [ 8].
Interestingly, RV contractility was reduced after ILO (slope of preload recruitable stroke work: 3.4 ± 0.8 vs 2.2 ± 0.5 mW/ml; P = 0.0002), while ventriculovascular coupling remained essentially preserved (ratio of RV end-systolic elastance over PA-Ea: 0.97 ± 0.33 vs 1.03 ± 0.15).
Interestingly, right ventricular contractility was reduced after iloprost-treatment (slope of preload recruitable stroke work: 2.2 ± 0.5 versus 3.4 ± 0.8 mWatt·s/ml; P = 0.0002), whereas ventriculo-vascular coupling remained essentially preserved (ratio of right ventricular end-systolic elastance to effective pulmonary arterial elastance: 0.97 ± 0.33 versus 1.03 ± 0.15).
The former, but not the latter, preserves ratios among pitches.
For example, if a circle has twice the diameter of another circle it will also have twice the circumference, preserving the ratio.
The stick figures were then standardized to a common height, preserving aspect ratio, and skeletonized to have 1 pixel line width.
The 89 samples, 39 of which metastasized, were randomly split into two halves preserving the ratio of metastasizing samples and each half was used to create two Golub-score ranked gene lists.
Size, 1280 X 720 HD, check Preserve Ratio box, in the drop down select Letterbox, OK.
Whenever this transformation is not distance-metric preserving the ratios will not be 1/2 and they will not co-vary.
Interestingly, both TIMP-1 and T2G mutant substantially inhibited STS-induced changes in the expression of Bcl-2 and Bcl-xL and Bax, preserving the ratios of Bax/Bcl-2 and Bax/Bcl-xL levels at basal control levels.
