The Ag/TiO2 protocol best preserved the cell wall structure, but cytoplasm leaking was observed.
The DIC image (Figure 7G) showed well preserved cell morphology.
The use of unfixed, live organisms is sometimes referred to as "ex vivo" fluorescence (evf) and is used because it preserves the live cell wall organization as opposed to cross-linking and other effects induced by fixation [27].
The ultrastructure of untreated yeasts was preserved, with compact cell wall (cw), nucleus (n), nucleolus (nu), mitochondria (m) and electron-dense glycogen granules (Fig. 3A).
Ag/TiO2 photocatalysis yielded almost 100%% inactivation efficiency and preserved the cell-wall shape of the spores, thus minimizing the biomolecule leakage.
The cross-wall is always formed de novo, but longitudinal cell walls of parental cells are preserved and become part of the daughter cell wall during vegetative cell division (Ettl, 1988 a, b; Massjuk, 1993; Van den Hoek et al., 1995).
Physiological characteristics of thermo-tolerant strains revealed that better cell wall integrity, higher trehalose content and enhanced metabolic energy were preserved by thermo-tolerant devices.
TEM images of untreated sugar cane bagasse clearly showed that the primary cell wall (PCW), secondary cell wall (SCW) and middle lamella (ML) were well preserved.
This cross-linking creates the semirigid gel properties characteristic of the cell wall matrix a process exploited in the preparation of jellied preserves.
Moreover, the cell wall gives them the capacity to resist digestion and pass through the gastrointestinal tract (GIT), and to be excreted in the faeces, while preserving their capacity to germinate and trap nematode larvae [ 2- 4].
