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DSCs accomplish this role by their unique asymmetric self-renewal, a cell division program that preserves stem cell phenotype with simultaneous production of cells committed to tissue-specific differentiation lineages.
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Minimal cytokine stimulation of HSCs may help preserve stem cell function and provide for the effective "activation" of stem cells that renders them susceptible to transduction.
The challenge of successful ex vivo expansion of HSCs is that the cells need to proliferate whilst preserving their stem cell properties: the ability to differentiate into all blood cell lineages and to undergo self-renewing cell divisions.
We also showed that MSCs could preserve their stem cell nature upon microencapsulation and easily be localized with retained viability upon in vivo implantation.
Cells were expanded (225-fold within 10 days) on nanofiber matrices preserving stem cell phenotype [26].
On the other hand, all these data show the need to develop novel culture protocols able to preserve stem cell plasticity.
Maintenance of the genome appears to be of utmost importance to preserve stem cell function and to reduce the risk of ageing associated dysfunctions and pathologies.
In conclusion, results provided by the present study suggest the need to develop novel culture protocols able to preserve stem cell plasticity.
This study implicates Notch signalling as a key pathway for preserving stem cell function in dystrophic muscles, and suggests that this pathway might be modulated to improve long-term satellite regenerative function in DMD.
Tissue-specific stem cells, which are capable of self-renewal to preserve stem cell pools as well as differentiation into a variety of effector cells, play a central role in the normal homeostatic maintenance and regenerative repair of tissues throughout the lifetime of an organism.
Serious cardiac side effects can result from the chemical used to preserve stem cells, making the transplant risky if a patient is unstable.
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