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While LGE enables precise assessment of transmural and even thin subendocardial scarring, simultaneous acquisition of sequential 13N-NH3 and 18F-FDG PET scans can identify and quantify myocardial areas with decreased perfusion or contractility, but preserved viability, so called hibernating or stunned myocardial tissue [52].
In the absence of cell cell and cell matrix contacts, encapsulated MIN6 β-cell survival diminishes within one week; however, in PEG hydrogel derivatives including the laminin sequences IKLLI and IKVAV, encapsulated β-cells exhibit preserved viability, reduced apoptosis, and increased insulin secretion.
Accordingly, studies in rodents have shown preserved viability and secretory activity after cerebral long-term implantation of micro-encapsulated cells [ 10, 23].
Starting from the global metabolism, we repeatedly deleted randomly chosen reactions from it, such that each deletion preserved viability, until we had arrived at a minimum metabolism, that is, a metabolism whose number of reactions cannot be reduced further.
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However, engineered tissues must have a functional vasculature immediately after implantation in order to preserve viability and function.
To preserve viability under chronic kidney disease induced stress, vascular smooth muscle cells increase exosome formation and release, but the result is aggravated pathological calcification.
Prior studies have shown that a two-pulse-electroporation protocol consisting of a short-duration, high-voltage first pulse followed by a longer, low-voltage second pulse can increase delivery efficiency and preserve viability.
In the BAU, water was used as the condensing vapor to preserve viability of virus, and supersaturation conditions for condensational growth of particles were achieved by either conductive cooling or mixing with hot, water-saturated air.
The SKC BioSampler collects particles in liquid, which helps preserve viability, but it does not collect small particles efficiently and is not size-selective.
In xav mutants, the deficiency of fatty acid metabolism and oxidative phosphorylation may force cells to augment glycolysis as an alternative energy source for energy and biosynthetic intermediates to preserve viability.
Here, we present a microdroplet based method to isolate and pattern single cells from heterogeneous cell suspensions (10% target cell mixture), preserve viability of the extracted cells (97.0±0.8%), and obtain genomic information from isolated cells compared to the non-patterned controls.
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