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In addition, chondrocytes proliferated on the scaffold and preserved their phenotype for up to 28 days.
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Cell-based therapies require removal of cells from their crowded physiological context and propagating them in dilute culture medium to attain therapeutically relevant numbers whilst preserving their phenotype.
They would allow a population of organisms (networks) to explore different regions of genotype space, preserving their phenotype while exploring many novel phenotypes.
All endothelial cell lines used in this study have been reported to preserve their phenotype for a limited range of passages (for hCMEC/D3: up to P33 [ 24], for hBMEC: up to P25, for TY10: at least up to P50 [ 34], and for BB19: up to P21 [ 27]).
Therefore, starting off with a limited number of NK cells would be of great interest for optimizing protocols for increasing the number of NK cells ex vivo by preserving their phenotypes.
The main challenge to harness the tolerogenic properties of DCs is to preserve their immature phenotype.
Glutamate-resistant HT22R cells were continuously cultivated in medium containing 10 mℳ glutamate to preserve their resistant phenotype.
Methodology for the cultivation of LEC on AM to preserve their stem phenotype and encourage proliferation and stratification continues to be rigorously investigated [5,10].
Thus, they preserved their traditions.
That will preserve their options.
This helps them preserve their privacy.
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