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In this table, the results are gathered in three sections: the reading order is preserved, the reading order is not preserved and all recordings together.
First mutations in the WD repeat domain all preserved the reading frame.
Motifs that frame-shifted their message had a significantly lower GC content that those that preserved the reading frame, at p-values less than 6e-12 in t-tests, regardless of mismatch level.
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Complementary oligonucleotides encoding the V5 epitope were designed in such a way as to generate SacII compatible termini following annealing (bold), and to preserve the reading frame when inserted into the SacII restriction site at nucleotide 123 within the 5' GS of the JV genome (underlined).
We found that indels supported by high quality sequence display a clear preference for multiple-of-three lengths (which preserve the reading frame), but this periodicity in the length distribution is almost completely erased by sequencing error in the raw 2× assemblies (Figure 4B).
Previous studies have also shown that ASEs possess several features that distinguish them from CSEs, such as weaker signals at alternative splicing sites, shorter lengths, higher level of sequence conservation, longer flanking intronic sequences, involvement of repetitive elements on exonization, and greater frequency for skipping exons to preserve the reading frame.
This situation is different for deleterious short-distance tandem splice sites that preserve the reading frame.
Next we checked the proportion of these possible protein-coding exons which preserve the reading frame.
Neither constitutively spliced nor RIs tend to preserve the reading frame.
Compensatory indels involve two or more indels that combine to preserve the reading frame.
Thus, the transcript will preserve the reading frame when the length difference of the two exons is an exact multiple of 3 nt in the ME group.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com