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In fact, as long as the linker peptide of GB2 is preserved, the activity of the dimer is maintained in a variety of chimeric combinations (Margeta-Mitrovic et al., 2001).
The DR-ultrafiltrate preserved the activity of BamHI exposed aerobically to 4 kGy, but PP-, EC- or TT-ultrafiltrates did not (Figure 1B).
When reconstituted in vitro at concentrations approximating those in the D. radiodurans cytosol, peptides interacted synergistically with Mn2+ and orthophosphate, and preserved the activity of large, multimeric enzymes exposed to 50,000 Gy, conditions which obliterated DNA.
When orthophosphate (13 mM) (Figure 2A), Mn2+ (200 µM) (Table 1), and peptides (3 mM) (calculated from Figure 4B, see above) were combined in vitro at concentrations approximating those in D. radiodurans, the mixture preserved the activity of BamHI and glutamine synthetase exposed to 17.5 kGy (Figure 4D, gels 3 5; and 4E), but did not significantly protect DNA (Figure 5).
As a result of their high homologies, most of the crossovers preserved the activity of the parents.
Similar(55)
HepG2 cells have preserved the activities of several phase I and phase II enzymes [ 28].
Coating with 52IPEG was the most efficient in preserving the activity.
It also creates a biologically friendly environment to preserve the activity of the enzyme.
The nanocomposites interface showed improved immobilization capacity for cells and good biocompatibility for preserving the activity of immobilized living cells.
The aim of this study was to design an optimized formula that preserves the activity of baculovirus stocks during prolonged periods of dehydration at various storage temperatures.
The methods were designed to preserve the activity of the immobilized protein such that the arrays provide a highly miniaturized platform to simultaneously interrogate the functional activities of thousands of proteins.
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