Specimens were snap-frozen under liquid nitrogen and subsequently stored at −80 °C for expression analysis or preserved in Tissue-Tek (Torrance, CA, USA) embedding medium and frozen for immunohistochemical studies.
Formalin-fixed, paraffin-embedded colorectal carcinoma tissue samples were preserved in the tissue archives of the Pathology Department at CGMH.
In contrast, insulin-induced autophosphorylation of the insulin/IGF-I receptor and phosphorylation of Ser473-Akt were preserved in adipose tissue and liver (Fig. 1 B and C, respectively), in which DMPK is not expressed.
The two in-vivo imaging areas, located in the superior retina were preserved in one tissue block.
The cellular integrity of the myocardium was well preserved in the tissue of hearts reperfused with IGF-1 (Fig. 1).
VEGF may exert a beneficial effect on podocytes; this is supported also by data showing that nephrin and CD2AP, target proteins regulated by VEGF, were partially preserved in renal tissue of MSC-treated animals [ 39].
These data suggest that signatures of primed, but not acute activated, microglia are preserved in brain tissue expression data from models of Alzheimer's disease, prion infection and, aging.
Monoallelic expression in all tissues is preserved up to e7.5, after which a high level monoallelic expression is preserved in extraembryonic tissues, while significantly reduced, but biallelic, expression in somatic tissues can be observed.
The ultrastructure of the oocytes was well preserved in vitrified tissues.
This observation was confirmed by TEM assesses which revealed that the organelles of granulosa cells and oocytes were well preserved in vitrified tissues.
