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Corals from recent collections were preserved in either >90% ethanol or at −80°C for genetic analysis.
Fish were preserved in either buffered formalin or ethanol.
White muscle (~200 mg) was sampled from each specimen and preserved in either DMSO solution (20% dimethylsulphoxide in saturated NaCl solution) or 70% ethanol solution.
Proglottids obtained from 4 case-patients in Shanghai were preserved in either 10% formalin (case-patient 16) or 70% ethanol (case-patients 17 19) after collection (Table).
Skin swabs were collected following the non-invasive procedure of Harlin et al. [ 35], preserved in either 90% ETOH or a solution of 20% dimethylsulfoxide (DMSO) saturated with salt, and stored at -20°C.
Skin samples were preserved in either RNA later® (Qiagen), DMSO 20% saturated with NaCl or in 70-96-100% ethatol at -20°C; blood samples were preserved in PAXgene™ (Qiagen).
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On the other hand, insulin dose response for GLUT4 translocation was largely preserved in cells expressing either Akt1W80A (EC50conMrol=0.100 nM; EC50MK-2206 treated=0.152 nM) or Akt2W80A (EC50conMrol=0.155 nM; EC50MK-2206 treated=0.256 nM; Figure 3F), indicating that both kinases are equally able to acutely regulate GLUT4 trafficking.
The penises are either preserved in formaldehyde and displayed in jars or are dried and hung or mounted on the walls of the museum.
Tumors were excised, halved, and either preserved in PFA or grown in cell culture media.
The specimens used were either preserved in 80% ethanol or air dried.
All other libraries were constructed by Amplicon Express (Pullman, WA) from either cells preserved in RNAlater (Ambion , Inc.
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