Three small branch tips (~1 cm) were taken from each colony using diagonal pliers, and preserved in DMSO preservation buffer (20% DMSO, 250 mM EDTA, NaCl saturated, pH 8.0; [ 63]).
Mononuclear cells were preserved in Buffer RLT and stored at –80°C; RNA was then extracted using the RNeasy Micro Kit from QIAGEN (Valencia, CA, USA).
More specifically, studies that have focussed on plant communities of boreal forests emphasize the importance of the width of the buffer strips [ 18, 19], and it has been stated that if the plant communities of the streams and the streamside riparian habitats are to be preserved, sufficient buffer strips are necessary [ 20- 22].
Biopsy specimen was preserved in buffered formalin solution.
Liver and kidneys were preserved in buffered formalin 10% for 24 hours for histological study.
Coral tissues were preserved in buffered guanidium thiocyanate solution [ 40, 41] and their DNA purified on an ABI Prism 6100 Nucleic Acid PrepStation.
Turf algae and symbiotic crustaceans were removed from the nubbins and a small piece of the nubbin was preserved in 20% DMSO preservation buffer for genotyping purposes.
Even under larger changes over evolutionary time, the tendency of the system to stay close to the valley floors will preserve and buffer developmental pathways in the face of evolutionary change.
Under ideal conditions, samples prepared for single-particle electron cryo-microscopy (cryo-EM) can be nearly perfect, in the sense that molecules of interest are surrounded by vitrified buffer, preserved in a life-like state.
In 1792 the French revolutionary government annexed the Austrian Netherlands (modern Belgium), which were traditionally preserved as a buffer state.
