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By anchoring the costamere, the principle adhesion structure along the length of the individual myofibres, stably to the ECM, DG serves to preserve membrane integrity by protecting the cell against contraction induced membrane damage.
This includes irreversible loss of plasma membrane potential, permanent acidification of the external plasma membrane/cytosol, and activation of the external membrane phospholipid scramblase system to attempt to preserve membrane integrity.
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Hypercapnic acidosis decreased 24-h stretch-induced interleukin-8 secretion (a, b), preserved membrane integrity as assessed by epithelial LDH leakage (c, d), and maintained cell viability (e, f) in human bronchial epithelial cells and BEAS-2B cells, respectively.
Figure 1A shows that the production of IL-8 secreted to culture supernatants by viable HT29 cells was reduced in 24 h by exposure to low concentrations cyclophosphamide, while cells still preserved membrane integrity (>90% viability by trypan blue exclusion).
A potential role for FGFs should not be discounted since they have important functions in embryonic prostate and embryonic epithelial morphology via cytoskeletal remodelling as well as in preserving membrane integrity [49], [50].
Exogenous application of polyamines led to, in varying degrees, preserved membrane integrity and lower growth inhibition during stress, reduced accumulation of ROS and increased activity of antioxidant enzymes such as catalase.
The possible mechanisms involved regard the improvement of cellular functions mainly aimed to preserve membrane function and integrity.
Nevertheless, GSH is also essential in preserving the ability of the cell to generate ATP and to maintain membrane integrity [ 13], and it has been suggested that GSH may protect cells by mechanisms independent of its antioxidant properties.
The phenomenon suggested that SVH could effectively maintain membrane integrity.
The experiments were designed to determine the extent to which SMM is able to preserve cell membrane integrity or reduce the degree of membrane damage in the course of low-temperature stress.
Thus PDGF-BB was able to preserve mitochondrial membrane integrity in human neurons subjected to Tat and morphine exposure.
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