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We have targeted the ATG-containing exon to preserve downstream regulatory elements (often found in the first intron) thus ensuring correct expression of reintegrated cDNAs.
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To preserve expression of downstream genes, the resistance cassette does not include transcription terminator sequences.
In the case of TssB and TssL, to preserve expression of the downstream gene, it was necessary to further fuse a second copy of the final nine amino acids of the original protein to the C terminus of mCherry or GFP.
The lowermost sequences are less well preserved at their downstream extremity and the whole system is both aggrading and prograding.
* The 'upstream' and 'downstream' indicate the numbers of frame-preserving upstream and downstream exons, respectively.
Thus, animal model systems in which animals are killed for sampling may be more suitable for RNA studies as mRNAs and ribosomes can be preserved rapidly for downstream analyses.
Cryptosporidium 18S rRNA gene qPCR was evidently more useful in process monitoring, with the pilot phase results showing that Cryptosporidium genome copies could more accurately be derived from these data, with the additional benefit of reduced analysis preserving material for downstream use.
Moreover, AMPK activity also acts on several downstream targets that preserve the energetic state of the cell and prevent liver I/R injury (reviewed in [ 46]).
While disputes over water have historically led to negotiated settlements over access, upstream countries will increasingly use dams and other projects "to obtain regional influence or preserve their water interests" over weaker countries downstream.
Biotinylated primers were bound to the beads in numbers small enough to preserve ample streptavidin binding sites for the downstream antibody binding step.
Furthermore, the data suggests that decreasing IL-6 induction of Nox2, or eliminating downstream superoxide production directly can preserve cognitive performance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com