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An advantage of the here-presented method is the use of mild conditions that preserve cell viability.
Regardless of the composition, the hydrogels were well able to preserve cell viability and proliferation as confirmed by in vitro cytotoxicity assay toward mesenchymal stem cells.
Spray drying is a less costly alternative to freeze drying in the mass-production of active dry microorganisms, if the drying conditions could be optimized to preserve cell viability.
Specifically, cellular exposure to a shear-stress impulse J≳0.1 Pa s ensures cell lysis or necrosis, whereas exposures in the range of 0.035≲J≲0.1 Pa s preserve cell viability while also enabling molecular delivery of 3 kDa dextran.
Although the time-course employed to study inflammatory responses in macrophages (up to 24 hrs) has shown to preserve cell viability and no toxicity was observable, the long term effect of noscapine and its analogs on macrophages is under current investigation in our laboratory.
Efficient cell cycle checkpoint signalling and DNA repair pathways have therefore evolved to preserve cell viability (Jackson and Bartek, 2009).
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Furthermore, changes in rhizosphere hydrophobicity by fatty acid exudates may be an important mechanism for preserving cell viability.
Various thiol-terminated polymers were covalently immobilized and unnatural functions were introduced to cell surfaces while preserving cell viability.
To that end, we optimized cultures of hRECs and showed that aggregation in three-dimensional spheroids significantly preserved cell viability, proliferation, and trophic factor secretion.
Direct overexpression of IκBα attenuates the deleterious effects of stretch in normocapnia-exposed epithelial layers, decreasing NF-κB activation (b), reducing epithelial LDH leakage (c) and preserving cell viability (d).
We observed that the composition of the buffer used for layer-deposition was crucial to preserving cell viability, e.g. potassium ions were preferred to sodium ions during the coating.
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