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While the stable power and use of freezers and refrigerators with battery backup solved the problem of storing perishable reagents and samples in the laboratory, it did not address the issues related to preservation samples collected in the field.
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There are several factors that can lead to such conflicting results of PIK3CA mutations between studies, such as geographical variation/influence, sample source preservation, sample size and methods used for DNA isolation.
The steps commonly encountered in any analysis are sampling, sample preservation, sample preparation, and finally the chemical analysis.
The checklist, known as Minimum Information for any (x) Sample (MIxS) (2), itemizes elements to report about samples, sampling conditions and preservation, sample processing and processes such as data acquisition and processing.
The sampling procedure and preservation of samples were done according to the Standard Methods for the Examination of Water and Wastewater (APHA 2005).
In summary, we find that for long-term preservation of samples, flash freezing is the preferred method.
Furthermore, Vanderford et al [13] tested the preservation of samples for the trace analysis of 27 compounds including estrogens.
The preservation of samples in the field, their transportation and analysis of major ions in the laboratory has been carried out using the standard recommended analytical methods (APHA 2005).
Preservation of samples in the absence of cryoprotectant resulted in variable changes in community composition.
After the initial preservation period, samples were taken from both the preservation solution and the pericardium for microbiological cultures [ 9– 13].
The quality of the DNA, used for later molecular analyses, is an important factor and depends on collection and preservation of samples prior to DNA extraction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com