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Thus, understanding the details of these two parameters of tissue processing epitope exposure and formaldehyde fixation begins to provide a basis for establishing conditions that optimize the quality of macromolecule preservation in tissue.
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Antigen preservation in tissues was confirmed by assaying sections from the same TMAs for expression of phospho-tyrosines by immunohistochemistry, using a monoclonal antibody to tyrosine-phosphorylated proteins (clone 4G10, 1 : 500, Millipore, Billerica, MA, USA).
The quality of morphologic preservation in the tissue array sections were assessed against the whole tissue sections of the same cases.
Defining blood modules and studying their preservation in brain tissue is beyond the scope of this article.
Primers amplifying β-actin mRNA were used as a positive control for demonstration of mRNA preservation in the tissue, and the ability to detect gene expression.
Determining the quality of preservation of macromolecules in tissue is important to avoid false negative and false positive results.
Essential to accurate characterization of the molecular phenotype of a tumor is determining the quality of preservation of macromolecules in tissue.
Furthermore, ZFP-VEGF treated animals showed significant improvements in tissue preservation and neurobehavioural outcomes.
Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique.
The morphometry was performed only on 24 rats (6 per group) because of technical problems in tissue preservation.
Results from these studies indicate that postnatal reconstitution of Shh signaling resulted in tissue preservation and repair, and that gene therapy with Shh not only prevented fibrosis, it also promoted angiogenesis and regional blood flow.
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