To compare the effects of dimethyl sulfoxide (DMSO), 1,2-propanediol (PROH), sucrose, trehalose, concentration of cryoprotectants, equilibration method, and postseeding freezing rate on doe rabbit ovarian tissue preservation after freezing, using fractional experimental design.
Since the first preservation by freezing in the 1960s the process has been performed only a few hundred times.
This approach was refined in the 1930s in Russia, and the subsequent development of methods for the cryopreservation (preservation through freezing) of semen led to the widespread use of artificial insemination in animals.
To document the first successful fertility preservation with freezing mature oocytes for a cancer patient.
The goal of this study was to investigate the effects that two common preservation methods (freezing wrapped in saline-soaked gauze and refrigerating ethanol fixed samples) have on bone mechanical properties in the context of an in vitro ribosylation treatment designed to modify mechanical integrity.
Two preservation methods (freezing and freeze-drying) and the influence of several protectants were investigated.
In this study we use earthworms, a key organism in soil research, to compare three different DNA preservation methods – freezing at −20 °C, storing in 75% ethanol, and freeze drying.
The obtained results might also have some value for the bone banks where the level of expression of BMPs could change after single steps of bone preservation as freezing, liophilisation, radiation sterilization etc.
Finally, the earthworm pieces were individually put into 1.5 ml reaction vials and subjected to one of three preservation methods: freezing (F), ethanol (E), or freeze dried (D), ensuring that each of the 5 different bleaching treatments was represented by 5 samples in each of the preservation methods.
The resection-to-preservation (freezing) time was kept to less than twenty minutes.
