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Other applications include the use of perfluorocarbon emulsions to augment tumour oxygenation to render them more sensitive to radiation and chemotherapy, to prevent or treat sequelae of air embolism, and finally to improve organ preservation for subsequent organ transplantation (referenced in [ 1]).
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A study comparing immediate snap freezing in LN2 with ethanol-fixation and RNAlater-preservation techniques for subsequent assessment of gene expression changes in cervical cancer showed RNA quality to be equivalent to or better in snap-frozen tissues than RNAlater- or formalin-fixed preserved tissues (50).
To investigate the most appropriate surgical technique for optimizing hemostasis and preservation of subsequent fertility after postpartum hemorrhage (PPH).
Further dissection of these factors in a more controlled setting will provide significant benefits to the preservation of DNA for subsequent testing.
(iii) The RLT buffer (Qiagen, Germantown, MD) was selected as the suitable cell lysing agent and for subsequent preservation of the biomolecules (including RNA) for long periods of time at ambient temperature without causing any comprehensible degradation (Additional file 3: Table S1).
All in vitro extraction solutions were refrigerated at 4°C for preservation and subsequent analysis by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) (U.S. EPA 2007e).
Ridge preservation following dental extractions is fundamental, preserving the ridge profile for subsequent implant placement and providing a sustained function and esthetics.
This clinical trial reports on the successful application of an in situ hardening biphasic alloplastic bone graft substitute for ridge preservation and subsequent implant placement in 15 healthy patients.
First, we determined the optimal basal medium for subsequent screenings for a preservation medium.
The preservation of these system properties is highly desirable for subsequent feedback design of the squared-down system.
The primary challenge with whole organ cryopreservation, as distinct from the preservation of cell suspensions or tissue fragments, is the crucial need to preserve an intact vascular system to allow for subsequent organ revascularization and function (Pegg, 2002, 2007).
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