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Turf algae and symbiotic crustaceans were removed from the nubbins and a small piece of the nubbin was preserved in 20% DMSO preservation buffer for genotyping purposes.
Both the coral and the water filter samples were suspended in 20% DMSO (final concentration) preservation buffer [82] and stored at −20°C until further processing.
Donor lungs were explanted according to a standard European explant protocol (Eurotransplant), using cold perfused with preservation buffer and stored inflated on ice until use.
Coral tissue was separated from the coral skeleton with a modified airgun attached to a SCUBA cylinder and subsequently stored in 20% DMSO preservation buffer and kept at −20°C until further processing.
Muscle tissue was prepared for mitochondrial respiration measurements as follows: after taking the muscle biopsy, the sample was stored in an ice-cold preservation buffer (10 mM Ca-EGTA buffer, 0.1 µM free calcium, 20 mM imidazole, 20 mM taurine, 50 mM K-MES, 0.5 mM DTT, 6.56 mM MgCl2, 5.77 mM ATP, 15 mM phosphocreatine, pH 7.1).
Blood was kept in a preservation buffer solution (Longmire buffer, [ 57]) that allows the storage of samples without refrigeration.
Similar(51)
Technological advances in RNA preservation buffers have made it feasible to study CRC tumor-specific RNA transcripts as stool biomarkers.
In the present study, we evaluated four commercially available RNA preservation buffers applied to whole blood samples in seven different carnivore species.
Solutions to this dilemma include immediate snap freezing of the sample in liquid nitrogen or dry ice [ 27, 28], or storage in special whole blood preservation buffers.
Using a sterile syringe, approximately 0.5 ml of whole blood was transferred into each of four tubes containing unique RNA preservation buffers.
One observation supporting this possibility was the high amount of coagulated blood sampled in RNA later®, a pattern not prevalent in any of the other preservation buffers storing the same blood volume from the same animals.
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