As a result of their acylation, Wnt proteins are very hydrophobic and require detergents for purification, which presents major obstacles to the preparation and application of recombinant Wnt proteins.
But the next five years, with a European referendum looming, problems in Scotland just begun, unfunded pledges to finance and major cuts to make, will present major obstacles to Cameron's hope that this 2015 election can prove a watershed in UK politics, keeping Labour out for a generation.
For some, the social and professional hierarchies still present major obstacles.
Serbia's incoherent international identity and pernicious historical legacies also present major obstacles to its EU membership aspirations.
Unfortunately, the genetic heterogeneity associated with MPS II and the private nature of many mutations present major obstacles to genotype phenotype correlation.
The difficulty and costs of keeping track of a necessarily very large cohort and facilitating follow-up of a sub-sample who become pregnant present major obstacles in designing a study with a capacity to collect information from women immediately preceding and after conception.
Previously, the size and awkwardness of computers presented the major obstacles to portability.
Specifically, the standardised mean difference presents two major obstacles to interpreting the apparent effects.
Based on the three cases, we argue that prohibitive laws and governments' reluctance to institute and implement comprehensive rights approaches to SRH, lack of political leadership and commitment to funding SRHR policies and programmes, and dominant negative cultural framing of women's issues present the major obstacles to operationalising SRH rights.
However, the gene encoding MSP-1 (msp1), which is present as a single copy per haploid parasite genome, is highly polymorphic [ 8] and thus, presents a major obstacle to effective vaccine development.
