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Each direction was presented 20 times in a randomized order leading to the total of 240 presentations (trials).
In Figure 1 A, we show the activity of an example cell from cat area 17 in response to 20 stimulus presentations (trials) of a drifting sinusoidal grating.
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Often, a very large number of stimulus presentation trials are required to fully identify the DSP of biological neurons.
Typically, 'blank' trials were alternated with stimulus presentation trials and a total of 40 trials comprised one imaging block.
Using sequential presentation trials, we detected either preference for gynochrome females (in Doniños and Laxe) or no preference (in Louro) in experienced males.
In four cases increases in AP firing were significant at the single-neuron level (p<0.05), despite the modest numbers of stimulus presentation trials and low firing rates (see 'Materials and methods').
The stimulation protocol used to investigate neurovascular coupling and somatosensory evoked field potentials consisted of 10 stimulation presentation trials with an intertrial interval of 70 s, each delivering a 15‐s train of electrical pulses (2 Hz, 0.2 mA, intensity, and 0.3 ms pulse width) after a 10‐s prestimulation baseline period.
They were placed again in the training context and received one VDS presentation (test trial) after a 5-min adaptation period.
The stimulus contrast and eye-of-presentation were randomly chosen for each presentation (trial).
To minimize the effect of sensory memory [24], [38], the stimulus contrast, duration, and eye-of-presentation were randomly chosen for each presentation (trial).
Subjects reported their perception through a three-alternative forced choice (the +45° grating, the −45° grating, or the fusion of two gratings) after each presentation (trial).
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