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The presentation of proteins on surfaces is fundamental to numerous cell culture and tissue engineering applications.
Hydrogels are facile architectures for the controlled presentation of proteins with far-reaching applications, from fundamental biological studies in three-dimensional culture to new regenerative medicine and therapeutic delivery strategies.
The appropriate presentation of proteins and their density at the polarised surfaces markedly impacts the physiology of the epithelial monolayer.
Cross-presentation of tumour-lysate is possible, i.e. the presentation of proteins or oligopeptides with HLA class-I-molecules (Bennett et al, 1997).
Precise control over the presentation of proteins and growth factors in hydrogel matrices is critical for mimicking the native cellular microenvironment and promoting cell substrate and cell cell interactions for tissue engineering and regenerative medicine applications.
To overcome such limitations in interpreting relative presentation of proteins, functional annotation clustering was used to identify biological processes overrepresented among the proteins detected in the enriched secretome fractions.
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Antigen-presenting cells, including B-cells, are required for presentation of protein antigens to naive T-cells which thereby stimulate secretion of different cytokines and activate B-cells to differentiate into antigen-specific Ig-producing plasma cells.
Simplified presentation of protein globules.
Potential of this structure in the presentation of protein E on the surface of E. coli through non-classical pathway was indicated by western blotting, SDS page and fluorescent microscopy techniques, similarly its effectiveness was compared with Lpp-OmpA system.
Here, I review recent progress in understanding the activation of naïve CD8+ T cells in vivo, with particular emphasis on cross-priming, the presentation of protein antigens acquired by dendritic cells from their environment.
While the presentation of protein drugs in form of nanoparticles is in general advantageous regarding correct biodistribution, this principle might not apply to brain targeting that is hampered by particular bio-physical barriers.
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