Whereas the use of recombinant soluble peptide-MHC (pMHC) molecules for identification of specific T cells has increased dramatically over the last years [ 1- 3], the reciprocal approach of using recombinant soluble TCRs (that is, lacking the transmembrane and intracellular part) for specific detection of peptide presentation and targeting to specific pMHC on cells has proven far more difficult.
We applied an innovative event-related fMRI-design by presenting target ramps at different velocities (5, 10, 15, 20°/s) with both continuous target presentation and intervals of target blanking.
This is a result of the sequence of the mental processes that caused by the sequence of presentation of cue and target in different deceptive procedures.
This study was conducted to investigate the effect of the order of presentation of cue and target on the processes of deception.
We decided to analyze the whole second before target presentation and not while or after target presentation, because a) the stimulus presentation induces an arousal and modifies the EEG and b) the subjects' reaction leads to artefacts in the EEG and is expected 300-500 ms after target presentation.
For the alpha filtered and non-filtered data 800 ms epochs were extracted from the data beginning 300 ms preceding visual target presentation and ending 500 ms after target onset.
For the slopes, the only retest reliabilities larger than.50 were found for hand and car targets in the blocked variant presentation and the face targets in the mixed variant.
Each trial began with the presentation of the central fixation cross, which remained throughout the entire trial, and after 2000 ms the central prime was presented one line above fixation for 150 ms. Prime and target presentation was separated by 100 ms (250 ms SOA).
Improvements in the delivery and presentation of target antigens are ongoing and include such strategies as continuous antigen administration by lymph node perfusion (Kundig, personal communication), direct targeting of APCs with recombinant Listeria monocytogenes that has been engineered to express tumor-associated antigens [ 15], and dendritic cell (DC) vaccines [ 16].
The experimental design allowed simultaneous presentation of target and distractor sound sources at different locations.
The computerized CPT involves the presentation of target and non-target stimuli.
