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Increased expression of these surface molecules may reflect a skewing toward an antigen presentation phenotype, including IgE-mediated antigen presentation, a pathway critical in allergic asthma pathogenesis (Kim et al. 2010).
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In my presentation I will suggest a pathway as to how to accomplish this.
In all cases the observed bias in activation of CD8+ T lymphocytes by exogenous antigen points to an alternative presentation pathway favouring association of pp65 epitopes with MHC I.
The chaperoned peptides can be channelled into the endogenous class I presentation pathway of a subset of professional antigen-presenting cells, which are then able to prime CD8-positive CTLs.
19, 20 The two pathways are not separated, and antigens that are mainly processed by one class of HLAs can be presented by the other via a cross-presentation pathway.
It is commonly believed that delivery of antigen into the class I antigen presentation pathway is a limiting factor in the clinical translation of DNA vaccines.
Many viruses, including several viruses of veterinary importance, have evolved astounding strategies to interfere with the MHC class I antigen presentation pathway, as a means of evading the CTL response of the host.
It seems that here too genes of the antigen presentation pathway have a major role.
Figure 3 depicts the two central pathways, i.e. the antigen presentation pathways, and the acute response pathway, and their relationships.
Since recombinant Gag/Δpp65-VLPs share the particulate nature with these antigens, a cross-presentation pathway might also exists for them as well as for pp65 associated to intact HCMV virions (present in the positive control).
Since the observed increase in T cell response was based on priming by γ-irradiated cells, it was possible that DCs play an important role in antigen uptake from the apoptotic cells for T cell priming via a cross-presentation pathway.
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